Sortex Demo

Elektrophorese-Technik

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Sample preparation with the Sortex Kit

Dez 17, 2008


	The different Barley and Wheat varieties are defined via their protein patterns. These socalled Prolamines, in the case of Barley the Hordeins (Hordeum vulgare), are lipophylic membrane proteins. This is the reason why the following procedure was chosen... To see the videos, the real player is needed To check your separation and your standarts: Barley Wheat

1 The single grain is crushed...

2 ..and remains in the cup..

3 ..water is pipetted to the grain..

4 ..water soluble parts are removed..

5 ..water soluble parts are pipetted out..

6 ..extraction buffer is pipetted..

7 ..Prolamines were extracted..

8 ..Prolamines were extracted..

9 ..unsolubilized parts are centrifuged..

10 ..and go to a new cup, ready!