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Elektrophorese-Technik

The gel Company
fluorotechnics

fluorotechnics companies

SDS-Electrophoresis

A denaturing electrophoresis for proteins with the following features:
1.  All proteins (acidic and basic) run towards the anode
2.  The proteins run according to their molecular mass
3.  Also lypophylic (non water-soluble) proteins run

Dez 17, 2008

sdsmol02

The SDS molecule

Your target molecules -- which method?

Range Molecule

Method

Pattern

Kit

Gel

Buffer

Res

AN

60 kD - 600 kD

SDS, 5%T

spreaded

1021-21

1001-18

1002-03

0.75 kD

2004

20 kD - 300 kD

SDS, 7.5%T

spreaded

1021-22

1001-08

1002-06

0.5 kD

2003

12 kD - 200 kD

SDS, 10%T

spreaded

1021-01

1001-01

1002-06

0.5 kD

2003

10 kD - 150 kD

SDS, 12.5%T

spreaded

1021-08

1001-13

1002-06

0.5 kD

2003

5 kD - 125 kD

SDS, 15%T

spreaded

1021-03

1001-21

1002-06

0.5 kD

2005

5 kD - 600 kD

SDS, 7.5 - 20%T

compressed

1021-20

1005-05

1002-06

0.5 kD

2003

Glycoproteins

SDS-Borate, 10%T
or 15%

spreaded

---

1001-01
1001-21

1002-03

0.75 kD

2004