SDS

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	SDSGels - BlotSlotsGels - CleanGels - SDS-Buffers


	Blotting of film-supported gels?


	[Blotting]

Jun 29, 2009


Ready-To-Use: Simply cut the Ready-To-Use gels out of their plastic bag and place them on the cooling plate of your horizontal chamber. Load your samples and start electrophoresis!


	SDSGels: are Ready-To-Use horizontal gels for horizontal SDS-electrophoresis. Tris-Tricine-Acetate buffer system. Gel-dimension: 125 mm x 250 0.43 mm. The 25S, the 52S and 103S gels are microtiterplate compatible (9 mm). Buffer see below. AN: SDSGel . NF= These gels are polymerized on non-fluorescent gel support.




		SDSGel 25S

	NF = non-fluorescent film support




	SDSGel 103S



	1DGel flatbed NF 12.5% 25S see also “Proteomics”

Kit: Name & Info shelf life:12 month	SDSGel/1DGel Kit (compl.with Buffers & Wicks)	Dimension Matrix	Samples Thickness	Used for: Range	Resolution (range)	Qty.	Code	Price* Price per Lane
SDSGelKit	10% 25S	25 x 12.5 cm 10%T	25 à 15ul 0.43 mm	SDS 15 -300 kD	500 D (15 - 200 kD)	5 gels	1021-01	EUR 201 EUR 1.60
SDSGelKit	10% 52S	25 x 12.5 cm 10%T	52 à 6ul 0.43 mm	SDS 15 -300 kD	500 D (15 - 200 kD)	5 gels	1021-02	EUR 201 77 cent
SDSGelKit	15% 25S	25 x 12.5 cm 15%T	25 à 15ul 0.43 mm	SDS 5 -125 kD	500 D (5 - 200 kD)	5 gels	1021-03	EUR 201 EUR 1.60
SDSGelKit	15% 52S	25 x 12.5 cm 15%T	52 à 6ul 0.43 mm	SDS 5 -125 kD	500 D (5 - 200 kD)	5 gels	1021-04	EUR 201 77 cent

1DGelKit flatbed NF	12.5% 25S	25 x 12.5 cm 12.5%T	25 à 15ul 0.43 mm	SDS, 1D-DIGE 10 -250 kD	500 D (15 - 200 kD)	5 gels	1021-23	EUR 214 EUR 1.71
1DGelKit flatbed NF	15% 25S	25 x 12.5 cm 15%T	25 à 15ul 0.43 mm	SDS, 1D-DIGE 5 -125 kD	500 D (5 - 200 kD)	5 gels	1021-24	EUR 214 EUR 1.71


	(1002-13) Buffers & Wicks for SDSGels consists of: 100 ml Cathode and Anode, 15 ml Sample Buffer, 15 ml Sample Diluter, 10 Electrode Wicks, Tris-Tricine buffer system


	BlotSlotGel: The SDS BlotSlots are used for test-stick production. 2 slots à 200 ul + 1 marker slot. Tris-Tricine-Acetate buffer system. AN 2001

Name & Info shelf life:6 month	BlotSlotGel	Dimension Matrix	Samples Thickness	Used for: Range	Resolution (range)	Gels	Code	Price*
BlotSlot	10%	25 x 12.5 cm 10%T	2 à 200 ul 2 marker. 0.65 mm	SDS-electrophoresis for test-stick prod 15 - 300 kD	500 D (15 - 200 kD)	5	1010-57	EUR 133
BlotSlot	12.5%	25 x 12.5 cm 12.5%T	2 à 200 ul 2 marker. 0.65 mm	SDS-electrophoresis for test-stick prod. 10 - 200 kD	500 D (10 - 200 kD)	5	1010-50	EUR 133


	The Electrodes: normally not necessary, recipe is in the manual

Buffers & Wicks BlotSlotGel
Shelf life: 1 year

1002-05

for 5 gels

EUR 49


	CleanGels are washed and dried horizontal gels. Gel-dimension 125 mm x 250 mm (normal size), 217 mm x 260 mm (large size) They must be rehydrated in a CleanPool before use. Choose your suitable buffer system. The 25 and the 52 slot gels are microtiterplate compatible (9 mm)! All horizontal gels can be cutted to half or third size or a size adapted to the number of samples!




			For rehydration: CleanPool

	CleanGels for SDS-Electrophoresis

Name & Info shelf life:1.5 years	CleanGel (Select a buffer system below)	Dimension Matrix	Samples Thickness	Used for: Range	Resolution (Range)	Quantity	Code No	Price* Price per Lane
CleanGel	10% 25S	25 x 12.5 cm 10%	25 à 15ul 0.43mm	SDS 12 kD -200 kD	500 D (12 kD-120 kD)	5	1001-01	EUR 108 86 cent
CleanGel	15% 25S	25 x 12.5 cm 15%	25 à 15ul 0.43mm	SDS 5 kD -125 kD	500 D (5 kD-75 kD)	5	1001-21	EUR 108 86 cent


	The SDS-Buffers: for the CleanGels above

Name & Info shelf life:1 year	Usage	Contents (for 5 gels)	Type pH	Code	Price*
SDS Buffer Neutral	typical SDS Electrophoresis 10kD-150kD	200ml Rehydration Buffer 100ml Anode and Cathode 5 Drying Cardboards, 12 Electrode Wicks	Special Buffer-System pH 7.0	1002-06	EUR 75
Borate SDS Buffer	large Proteins & Glycoproteins 15kD-350kD	250ml Rehydration Buffer 250ml Electrode Buffer 5 Drying Cardboards, 12 Electrode Wicks	Tris/Cl - Tris/Borate pH 8.5	1002-03	EUR 54


	* T h e t a x - a n d t r a n s p o r t - c o s t s w i l l b e c h a r g e d a d d i t i o n a l l y !