Elektrophorese-Technik

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After the IEF first a simple contact-blotting is done.
A Nitrocellulose membrane 0.45 µm is pressed on the gel's surface (with some papers behind) for 30 min.
Then the Immuno-Staining with an Anti-human IGG Antibody (HRP-conjugated, DAKO P0449) is performed on the membrane.

Advantage: A well introduced technique, IGG-selective staining, no additional hardware.
Disadvantage: A additional technique (the blotting) has to be perfromed.

Gel: FocusGel 6-11 24S
Visualization: Immuno-staining after contact-blotting
(Courtesy of .A.W. Teelken UMCG, Neurologic Klinic, Groningen)