ETC-Logo

Elektrophorese-Technik

a fluorotechnics company

fluorotechnics
webapplication

All our Application Notes can be downloaded as pdf-files for free.
To reproduce these files on your computer you should use the Acrobat Reader. You can download it using the link on the right.

General Electrophoresis

Okt 10, 2008

getacro

P 01       Why should I use horizontal electrophoresis?
P 07     New Horizontal Chamber: FlatTop II
P 02      Why should I use washed and dried gels?
P 03     Cutting CleanGels to half Gels

Visualizations

AN 1028  Quantitative DNA-Fragment Electrophoresis using Direct Staining
        with the dye “Stains All”.
AN 1026  Fluorescence visualization of DNA-fragments using the stains GelStar and Sybr Green II
AN 2021  Staining with AzurGel Super (Coomassie-staining without destaining)
P 08     Use of Farmer's Reducer (recycling of silver-stained gels)
P 10     Immuno-Fluorescence Visualization of IgG on FocusGel 6-11

Blotting

New!

AN 3001   Blotting of Horizontal Gels. General manual.
BlotGel    Direct Blotting using Ready-To-Use BlotGels

Proteomics

                                          (horizontal)
AN 3003    Second dimension on 2DGel flatbed fluor
AN 4001    FlatTop Tower  A System for Multiple FlatBed Electrophoresis
                      
(vertical)
AN 3002    Second dimension on 2DGel fluor

DNA - Electrophoresis

                                                           Double stranded DNA
AN 1001      DNA-Mapping using Polyacrylamide Gel Electrophoresis in CleanGels with fast
                  and highly sensitive Silver Staining (Agarose <-> Polyacrylamide)
DNAGel      Ready-To-Use gels for DNA-fragments: DNAGels
AN 1018    Our best combination: CleanGel DNA-HP & Delect-Buffer
                 followed by a special silver-staining (Benzolsulfonic acide)
AN 1019      RAPD (Random Amplified Polymorphic DNA) analysis may provide an
                  
effective means to type bacterial strains.
AN 1007     Apolipoprotein E Genotyping on CleanGel 15%
                 Detection of small DNA-Fragments, PCR followed by RFLP

                                                  Single stranded DNA
AN 1022     Detection of Point Mutations with the new CleanGel SSCP plus
AN 1011     Strategy of SSCP-Electrophoresis
AN 1010     p53 Mutation Screening in Solid Tumors
AN 1020     SSCP Screening of HLA Genes using CleanGels and Delect-Buffer
AN 1021     Heteroduplex (Mismatch) Analysis with CleanGels and Delect-Buffer:
                 Analysis of HIV env Genes
AN 1027     Quality Control of Single Stranded DNA-Primers and Microsatellites

                                                 Length Polymorphism
AN 1017     Strategy of Microsatellite Analysis
AN 1015     Native and Denaturing Detection of Microsatellites using the
                 CleanGel HyRes plus System
AN 1012     Denaturing DNA-Electrophoresis on CleanGels
AN 1024     Gapex Polymer. Spreading up your Electrophoresis between 50 bp and 350 bp

                                           Differential Display and DGGE
AN 1016     REN (Rapid, Efficient and Nonradioactive) Display of DDRT-PCR Products
                  on CleanGel 15%T with 7M urea
AN 1009    Native Electrophoresis with DDRT-PCR samples
                  on CleanGel Long-10 and Large-10
AN 1023    Denaturing Gradient Gel Electrophoresis (DGGE)

                                                   Special
AN 1003     Semi-disc Electrophoresis of very small DNA-Fragments (+Visualization)
AN 1004     1 kb - 5 kb Separation using CleanGel 10%
AN 1002     103 DNA-Samples run in one Gel!
AN 1027    Quality Control of DNA-Primers, Oligonucleotides and Microsatellites

Protein - Electrophoresis

                                            SDS-Electrophoresis
SDSGel      Ready-To-Use Gel System for SDS-Electrophoresis: SDSGels
P 05           Our new SDS Buffer-Family (Includes “Hot Coomassie-Staining” Procedure)
AN 2003     SDS-Electrophoresis with CleanGels using the SDS Buffer Kit Neutral
AN 2005     SDS-Electrophoresis with CleanGels using the SDS-Peptide Buffer
AN 2004     SDS-Electrophoresis with CleanGels using the Borate-SDS Buffer Kit
AN 2014     SDS-Electrophoresis with Urinary Proteins
AN 2002     SDS-Electrophoresis of Milk and Soya-Products
AN 2012     Electrophoresis of Potato-Proteins
AN 2014     SDS Electrophoresis of Urinary-Proteins
AN 2001     Immunological Test-Stick Production using WetGel-BlotSlot
AN 2020     SDS-Electrophoresis and Immunodetection of Factor VIII proteins

                                     Native Electrophoresis
AN 2009     Native, acidic Electrophoresis of Membrane Proteins
AN 2008     Fast Analysis of Barley-, Wheat-, Mellit-, Rice- and Maize-Prolamines (Hordeins)
         using the SORTEX-Kits 10% and 15%
AN 2012     SDS and native Electrophoresis of Potato-Proteins
AN 2022     Blue-Native Electrophoresis of Plant Leaf-Extracts
AN 2024     Native Electrophoresis of Tomato Leaf-Extracts
AN 2012     Electrophoresis of Potato-Proteins

                                             Isoelectric Focusing
AN 2006     Urea- and Native Focusing using CleanGel IEF Ultra
FocusGel    Isoelectric Focusing on Ready-To-Use FocusGels 3 - 10
AN 2007     Detection of Cows Milk-Caseins in Ewes and Goats-Milk Cheese
AN 2010     Detection of Normal and Pathological Hemoglobin by Isoelectric Focusing
         on CleanGels and FocusGel 6-9 24S
AN 2018     Isoelectric Focusing of Serum and Cerebrospinal Fluid using  FocusGels 6-11 24S
P 09      Oligoclonal IGG-pattern in IEF of CSF and Serum. A comparison between
         Agarose- and PAG-gels
P 10      Immuno-Fluorescence Visualization of IgG on FocusGel 6-11
AN 2023     Isoelectric Focusing using CleanGel IEF Bidirect with Slots
AN 2012     Electrophoresis of Potato-Proteins